Figure 5
From: Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP
Analysis of the conserved cysteine in CCP2. Position of the cysteine in the apo- (A) and holo- (B) structural models of CCP2 dimer. (C) Absorption spectrum of the holo-CCP2 with different concentrations of DTT. (D) Analysis of the presence of the disulfide bond by SDS-PAGE with and without reductant. Numbers on the left correspond to the molecular weight marker (lane 1) in kDa. (E) P(r) functions obtained for the SAXS profile of apo-CCP2 measured in solution with (5 mM TCEP) (green line) and without reductant (red line) in comparison to the holo-CCP dimer (blue line). (F) P(r) functions obtained for the SAXS profile of holo-CCP2 measured in solution with (green line) and without reductant (blue line) in comparison to the apo-CCP dimer (red line). Experimental SAXS profiles used to calculate P(r) functions are shown in the Figure S4.
