Figure 2

Effects of hmxbato on the proliferation and recovery of tumor and normal lung cells. Tumor (A549) and normal (BEAS-2B) lung cells were incubated for 24 h in the absence (control) or presence of different concentrations of hmxbato (0.95 µM, 1.9 µM, 3.8 µM, 19 µM and 38 µM). The proliferation of A549 (A) and BEAS-2B (C) cells was evaluated after 24, 48 and 72 h of treatment. Recovery from the cell proliferation induced by the hmxbato treatment was also assessed. For that, A549 and BEAS-2B cells treated with complete medium (control), FBS free medium and different concentrations of hmxbato (0.95 µM, 1.9 µM, 3.8 µM, 19 µM and 38 µM) for 24 h were washed with PBS and incubated with complete medium. The recovery of A549 (B) and BEAS-2B (D) cells was evaluated at 0, 24 and 48 h. Data were expressed as mean ± standard deviation of experiments performed in triplicate. Three independent experiments were performed. Statistical differences were determined using one-way ANOVA and Tukey's multiple comparison test. Significant differences with respect to control were considered when p < 0.1 (*), p < 0.01 (**), p < 0.001 (***) and p < 0.0001 (****).