Figure 2 | Scientific Reports

Figure 2

From: Ruthenium (II) complex cis-[RuII(ŋ2-O2CC7H7O2)(dppm)2]PF6-hmxbato induces ROS-mediated apoptosis in lung tumor cells producing selective cytotoxicity

Figure 2The alternative text for this image may have been generated using AI.

Effects of hmxbato on the proliferation and recovery of tumor and normal lung cells. Tumor (A549) and normal (BEAS-2B) lung cells were incubated for 24 h in the absence (control) or presence of different concentrations of hmxbato (0.95 µM, 1.9 µM, 3.8 µM, 19 µM and 38 µM). The proliferation of A549 (A) and BEAS-2B (C) cells was evaluated after 24, 48 and 72 h of treatment. Recovery from the cell proliferation induced by the hmxbato treatment was also assessed. For that, A549 and BEAS-2B cells treated with complete medium (control), FBS free medium and different concentrations of hmxbato (0.95 µM, 1.9 µM, 3.8 µM, 19 µM and 38 µM) for 24 h were washed with PBS and incubated with complete medium. The recovery of A549 (B) and BEAS-2B (D) cells was evaluated at 0, 24 and 48 h. Data were expressed as mean ± standard deviation of experiments performed in triplicate. Three independent experiments were performed. Statistical differences were determined using one-way ANOVA and Tukey's multiple comparison test. Significant differences with respect to control were considered when p < 0.1 (*), p < 0.01 (**), p < 0.001 (***) and p < 0.0001 (****).

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