Figure 3 | Scientific Reports

Figure 3

From: Ruthenium (II) complex cis-[RuII(ŋ2-O2CC7H7O2)(dppm)2]PF6-hmxbato induces ROS-mediated apoptosis in lung tumor cells producing selective cytotoxicity

Figure 3The alternative text for this image may have been generated using AI.

Hmxbato prevented the clonogenic growth of lung tumor cells. Tumor (A549) and normal (BEAS-2B) lung cells were incubated for 24 h in the absence (control) or presence of hmxbato (1.9 µM, 3.8 µM and 19 µM). (A) Representative images of an experiment developed in 6-well plates showing the formation of A549 and BEAS-2B cell colonies after removal of treatments and incubation with culture medium for 14 days. (B) Bar graphs showing plating efficiency (%), calculated by the number of colonies formed / number of cells seeded × 100%. Data were expressed as mean ± standard deviation of experiments performed in triplicate. Three independent experiments were performed. Statistical differences were determined using one-way ANOVA and Tukey's multiple comparison test. Significant differences with respect to control were considered when p < 0.1 (*), p < 0.01 (**), p < 0.001 (***) and p < 0.0001 (****).

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