Figure 5 | Scientific Reports

Figure 5

From: Ruthenium (II) complex cis-[RuII(ŋ2-O2CC7H7O2)(dppm)2]PF6-hmxbato induces ROS-mediated apoptosis in lung tumor cells producing selective cytotoxicity

Figure 5The alternative text for this image may have been generated using AI.

Hmxbato induced apoptosis in lung tumor cells. Tumor (A549) and normal (BEAS-2B) lung cells were incubated for 24 h in the absence (control) or presence of hmxbato (3.8 µM and 19 µM) and subjected to annexin V-FITC and propidium iodide double-staining assay. (A) Dot plot graphs, referring to one of the independent experiments performed, showing the distribution of A549 and BEAS-2B cells in different quadrants, according to the incorporation or not of Annexin V (AV) FITC/propidium iodide (PI). Lower left quadrant, viable cells; right lower quadrant [AV(+)], initial apoptosis; right upper quadrant [AV/PI(+)], late apoptosis; upper left quadrant [PI(+)], necrosis. (B) Bar graphs showing the quantification of A549 and BEAS-2B cells in apoptosis/necrosis. Data were expressed as mean ± standard deviation of experiments performed in triplicate. Three independent experiments were performed and over 10,000 cells were analyzed in each replicate. Statistical differences were determined using one-way ANOVA and Tukey's multiple comparison test. Significant differences with respect to control were considered when p < 0.1 (*), p < 0.01 (**), p < 0.001 (***) and p < 0.0001 (****).

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