Figure 8
Hmxbato induced mitochondrial damage in lung tumor cells. Tumor (A549) and normal (BEAS-2B) lung cells were incubated for 24 h in the absence (control) or presence of hmxbato (3.8 µM and 19 µM) and stained with rhodamine 123 to detect changes in the mitochondrial membrane potential (∆Ψm). The fluorescence intensity of A549 (A) and BEAS-2B (B) cells was quantified by flow cytometry and presented in bar graphs. Data were expressed as mean ± standard deviation of experiments performed in triplicate. Three independent experiments were performed and over 10,000 cells were analyzed in each replicate. Statistical differences were determined using one-way ANOVA and Tukey's multiple comparison test. Significant differences with respect to control were considered when p < 0.1 (*), p < 0.01 (**), p < 0.001 (***) and p < 0.0001 (****).
