Figure 3

Comparison of enzyme substrate conversion in the renal cortex and medulla region of selected enzymes with regional differences. The spectral mass intensity dot plots and their corresponding box plots in the left columns represent the cortical region and those in the right columns the medulla as shown in (E). The first rows (S) represent the time-dependent intensities of the substrate masses and the second (and third) rows the corresponding product intensities (P). Enzyme substrate peptides are (A) #6 MMP-2, (B) #7 caspase, (C) #19 PTP1B, TC-PTP, SHP-2, and (D) #20 protein tyrosine phosphatase. (E) Representative H&E stain of a kidney section (after 5 min of incubation) with ROIs used for quantifications in (A) to (D).