Figure 2
From: Live-cell imaging with Aspergillus fumigatus-specific fluorescent siderophore conjugates
(A) Uptake of different radiolabelled, fluorescent conjugates normalized to uptake in iron-depleted media of the respective compound. Grey bars represent control conditions with TAFC. Blocking with [Fe]TAFC reduces cellular uptake due to competition with the natural substrate of the MirB transporter. Fe (+) represents iron-replete conditions, which decreases biosynthesis of the MirB transporter. (B) Competition assay of [68Ga]Ga‐TAFC blocked with iron-containing fluorophore compounds in iron-depleted minimal medium. Values that are lower compared to the [68Ga]Ga‐TAFC uptake of control (grey bars), indicate a specific interaction of the fluorophore conjugate with the MirB transporter. Data of [Fe]DAFC-Cy5 are adapted from reference18.
