Figure 5
From: Live-cell imaging with Aspergillus fumigatus-specific fluorescent siderophore conjugates
Co-staining with FM1-43 (green, mitochondria) and DFFDA (hot orange, vacuoles) confirmed the subcellular accumulation of different siderophore conjugates to fungal mitochondria and vacuoles, respectively. Scale bars, 10 µM. Supplementary Video S1 shows a time-lapse sequence of [Fe]DAFC-Cy5/FM1-43 co-labelling and supplementary Figure S2 shows Calcolfuor White/FM1-43 co-staining.
