Figure 2

Effects of C5a on neutrophil function: transmigration and respiratory burst. (A) Transmigration is expressed as the number of cells counted per high power field. (B) Respiratory burst is expressed as the amount of superoxide release per million cells. Data are expressed as the mean ± SEM of 4 separate donors. Differences between groups were calculated using ANOVA with a Tukey’s post-hoc test where p < 0.05 considered significant. *Represents a significant difference between neutrophil transmigration with and without IL-8. **Represents a significant difference between IL-8 stimulated neutrophils treated with and without C5a. #Represents a significant difference in respiratory burst between neutrophils stimulated with PMA and those without PMA.