Figure 5 | Scientific Reports

Figure 5

From: A computational guided, functional validation of a novel therapeutic antibody proposes Notch signaling as a clinical relevant and druggable target in glioma

Figure 5

Phenotypic characterization of BRON treatment. (A) Mildly (GBM1) or no (407p) effect on cell growth was observed whereas significant reduction in cellular invasion was observed upon long-term exposure to the drug (day 6 treatment). (B) Genetic suppression of Notch1 by shRNA did only in part pheno-copied the anti-invasive effect of BRON treatment. (C) Exposure of the cells to GSI MRK003 led to reduction of cell invasion in GBM1, which was severely lower as observed under BRON treatment, and no effect in 407p. Validation of previously established MRK003 treatment protocol28, 43 see Suppl. Figure S2. Scan of original Western blot membrane can be found in Suppl. Figure S8.

Back to article page