Figure 4
From: Direct visualization of the extracellular binding structure of E-cadherins in liquid
AFM images of the dynamic changes E-cad-Fc. (a) AFM image of E-cad-Fc in a PBS solution containing 0.9 mM Ca2+ before the addition of chelating agent, EDTA (scale bar 50 nm). It shows an EC domain with a rod-like structure, a trans-binding portion of the EC1 (pointed by white arrow) and an Fc domain. (b)–(d) The continuously obtained AFM images about 0, 3, 6, 18 min after addition of EDTA (scale bar 20 nm). (e,f) AFM images of E-cad-Fc 18 min after addition of EDTA (scale bar 20 nm and 50 nm respectively). (g) AFM image of E-cad-Fc in a PBS solution without Ca2+(scale bar 50 nm).
