Figure 6

Neutralization of pseudoviruses expressing Lassa virus glycoprotein complex representing lineages II, III and IV by plasma or serum samples of Lassa fever survivors. 50% reciprocal neutralization titres of serum or plasma from Nigerian and Sierra Leonean Lassa fever survivors were determined using LASV pseudoviruses (LASVpv) expressing LASV glycoprotein complexes representing lineages II–IV (LII, LII, LIV). Panel (A): Lassa fever survivors from Nigeria (n = 37). Panel (B): Lassa fever survivors from Sierra Leone (n = 39). Note that not all samples from the cohorts had sufficient volume to perform the three pv assays. Panel (C): Mean of the 50% reciprocal neutralization titres represented in panels (A,B). Panel (D): neutralization curve for Nigerian subject 2 (NG2). Panel (E): neutralization curve for Sierra Leonean subject 20 (SL20). Blue bars or symbols are pp representing lineage II. Red bars or symbols are pv representing lineage III. Green bars or symbols are pv representing lineage IV. Data was analyzed using Microsoft Excel (version 16.39, Microsoft, Redmond, WA), JMP software (version 13.0.0, SAS Institute, Inc., Cary, NC) and Prism (version 6.07, GraphPad Software, Inc., San Diego, CA). The figure was compiled using Adobe Illustrator (version 15.1.0, San Jose, CA).Error bars represent standard error of the mean, which is smaller than the symbols in some cases. Asterisks in panels (D,E) represent enhancement at the indicated dilutions.