Figure 1

Study design and body temperature in SARS-CoV-2 infected tree shrews. (a) Total 35 tree shrews (Tupaia belangeris) were used for infection in this study. Two challenge experiments were conducted with 1 ml 10⁶ pfu SARS-CoV-2 nasally (500 μl/each). Twenty-four animals for the first experiment were divided into three groups (young, adult and old) according to ages. Each group included half male and half female. Following the viral inoculation, clinical observation and viral load assay were performed as indicated. Animals were dissected in about two weeks post viral inoculation. (b) On every other day as indicated in (a), body temperature of tree shrew was monitored and recorded. The software Graphpad was utilized for data processing and plotting as rainbow heat map. X represents no data collected. Body temperature beyond 39 °C was shown in white boxes.