Figure 3
Effect of RNAi suppression of DvABCB1 on D. virgifera virgifera larvae exposed to WCR toxins. WCR neonates were treated with test dsRNA samples [100 µg/mL in diet or water (control)] for 2 days and transferred to the same treatments that were freshly prepare for two more days. Treated larvae were then exposed to protein toxins at the doses equivalent to LC80-85. The effects of treatments on WCR larvae were scored 14 days after initial treatments. Least square means pairwise comparison p values: > 0.05 ns (not significant), < 0.0001***. (a) Gene suppression analysis of WCR larvae 4 days after dsRNA treatment [mean ± SE; n = 9–12 per treatment (5–6 insects per treatment and n = 2 PCR sub samples per insect)]. Relative expression of DvABCB1 by qRT-PCR assay is shown for each treatment using DvRPS10 as a reference and normalized to DvABCB1 expression in water control. F (2, 30) = 12.37, p value < 0.001. Least square means pairwise comparison p values: > 0.05 ns (not significant), < 0.001***. (b,c) Exposure to IP3-H9 after dsRNA treatment: Mortality (F (5, 66) = 15.88, p value < 0.001, and growth inhibition (F (5, 66) = 20.89, p value < 0.001). (d,e) Exposure to IPD072Aa after dsRNA treatment: Mortality (F (5, 66) = 19.54, p value < 0.001, and growth inhibition (F (5, 66) = 27.34, p value < 0.001).
