Figure 4
Effect of RNAi suppression of DvABCB2 on D. virgifera virgifera larvae exposed to IP3-H9. WCR neonates were treated with test dsRNA samples [100 µg/mL in diet or water (control)] for 2 days and transferred to the same treatments that were freshly prepare for two more days. Treated larvae were then exposed to IP3-H9 at 200 µg/mL in diet. The effects of treatments on WCR larvae were scored 14 days after initial treatments. Least square means pairwise comparison p values: > 0.05 ns (not significant), < 0.0001***. (a) Mortality (F (7, 56) = 20.15, p value < 0.001, percent mortality data was analyzed using general linear mixed model with Gaussian distribution with identity function and the Laplace likelihood approximation method. (b) Growth inhibition (F (7, 56) = 9.90, p value < 0.001). Growth inhibition (dead plus severely stunted larvae/total exposed) data were subjected to statistical analysis using general linear mixed model with a binomial distribution, a logit link function, and the Laplace likelihood approximation method.
