Table 1 Oligo Sequences for dsRNA Synthesis by IVT.

From: Functional validation of DvABCB1 as a receptor of Cry3 toxins in western corn rootworm, Diabrotica virgifera virgifera

Name

Oligo designation

Sequence

DvABCB1

5′ external forward

TAATACGACTCACTATAGGGTGACAGAAGAAAAAAAACATAGTATAAAGGATAAAGAGAA

Internal reverse

TCCTTTGGTTCTTCACTATTAACAAATTGGGCATCAATACCAATTTTCTCTTTATCCTTT

Internal forward

TGAAGAACCAAAGGAAAAAATTAAGAATGTATCTTTTCCTCAGATGTTTAGGTATGCAAG

3′ external reverse

TAATACGACTCACTATAGGGTACCATTAAAAATTTATCATAAGTACTTGCATACCTAAAC

DvABCB2

5′ external forward

TAATACGACTCACTATAGGGAACTCTATCGGCAGTAATCACTGGTTGTCTACCACCAATA

Internal reverse

ATATTGCACAGCATTTCCTGCAAGTTCTCCGAATAAAATTGTATTTATTGGTGGTAGACA

Internal forward

AATGCTGTGCAATATGCTGAAACTTTATATAACGCTACCTTATCACAAAACGAACAAGCT

3′ external reverse

TAATACGACTCACTATAGGGCATCAAAGAATTTTTCCTGAGCCTCAGCTTGTTCGTTTTG

GUS

5′ external forward

ATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCAAAA

Internal reverse

CCACAGTTTTCGCGATCCAGACTGAATGCCCACAGGCCGTCGAGTTTTTTGATTTCACGG

Internal forward

ATCGCGAAAACTGTGGAATTGATCAGCGTTGGTGGGAAAGCGCGTTACAAGAAAGCCGGG

3′ external reverse

TTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTA

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