Figure 4

Spatial probability-distribution maps. Upper row: control-group representative maps. The influence of 3T3 S, Caco2, 3T3 NIH and H9c2 (2-1) cells on the spatial distribution of trypomastigotes is illustrated in the images on the second, third, fourth and fifth rows, respectively. For the experimental group maps, the first, second and third columns respectively corresponds to low (\(\sim \)25%), medium (\(\sim \)50%), and high (\(\sim \)100%) confluence experiments. Dark-shaded areas correspond to the surface occupied by the cells in the experiments of low and medium confluence. No dark-shaded areas are shown in the third column maps because, in the high-confluence experiments, the entire field is covered by cells. This figure was elaborated employing the software MATLAB R2018a/URL: https://www.mathworks.com/products/matlab.html.