Figure 1

Biological sex has no detectible effect on CSF influx regardless of anesthetic. (a) Min/max boxplot of average intensity per animal for male and female mice under six different anesthetics. Individual animals are represented by single, colored dots. Red: female, blue: male. Isoflurane + dexmedetomidine: ISODEX, ketamine/xylazine: KX. (b) Anterior to posterior coronal section analysis (top), and residual intensity plots for all mice in every group across six coronal sections (bottom). Residual = (single animal mean fluorescence – mean anesthetic group fluorescence)/total variance in anesthetic group. Single animals are represented by dots. Group mean indicated by solid line. Standard deviation shown by shading. Red: female, blue: male. n = 68 female and 81 male mice (c) Mean intensity of fluorescence across 6 coronal sections in male and female mice under six different anesthetics. Mean and standard deviation shown with solid line and shading. Asterisk indicates 2-sided t-test with Bonferroni adjustment: p = 0.015. Individual mice shown by colored dots. Pentobarbital: n = 12 female and 15 male mice; α-chloralose: n = 12 female and 8 male mice; Avertin: n = 13 female and 14 male mice, ISODEX: n = 5 female and 9 male mice; Isoflurane: n = 9 female and 15 male mice, KX: n = 16 female and 20 male mice. Slice cartoons in (b) were created by Dan Xue within the Nedergaard lab.