Figure 5
From: PM014 attenuates radiation-induced pulmonary fibrosis via regulating NF-kB and TGF-b1/NOX4 pathways
Effect of PM014 on IR-induced apoptosis. (A) Representative TUNEL staining (green) of irradiated mouse lung tissues. Magnification, × 400 (Scale bar 50 μm). The treatment groups were Control (untreated); IR, (75 Gy irradiation); IR + PM014 (IR + 200 mg/kg PM014); PM014 (200 mg/kg PM014 only). Immunohistochemistry (B) and western blotting (C) using irradiated mouse lung tissues. Magnification, × 400 (Scale bar 100 μm). Full length blots are presented in Supplementary Fig. S9. The treatment groups were Control (untreated); IR, (75 Gy irradiation); IR + PM014 (IR + 200 mg/kg PM014); PM014 (200 mg/kg PM014 only). (D) The pro-apoptotic protein Noxa (green) was co-stained with pro-SP-C (red), a marker for type II AECs, using irradiated mouse lung tissue. Magnification × 400 (Scale bar, 50 μm).
