Figure 4 | Scientific Reports

Figure 4

From: Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides

Figure 4

Chemical shift and broadening maps for KS and KSDS binding to Gal-7. Chemical shift (∆δ vs. amino acid sequence with the horizontal black line indicating 1SD above the average value) and resonance broadening (∆Intensity or ∆INT vs. amino acid sequence) maps derived from HSQC spectra of 15N-labeled Gal-7 (20 μM) (not shown) are presented in (A) and (B) in the presence of KS (5 μM) to Gal-7, and in (C) and (D) in the presence of KSDS (50 μM) to Gal-7. In the broadening maps, a value of 1 indicates that the resonance associated with that particular residue is no longer detectable, and a value of zero indicates no change in resonance intensity. (Insert to Fig. D) The average ∆δ values from HSQC of 15N-labeled Gal-7 are plotted vs. the KS or the KSDS concentration (μM) as indicated. Solid lines show exponential fits to these data.

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