Table 1 Primers, probes and references for PCR and qPCR assays used in this study. All the assays had efficiencies within 100% ± 5% and an annealing temperature of 60 °C.

From: A non-lethal method for detection of Bonamia ostreae in flat oyster (Ostrea edulis) using environmental DNA

Gene

Organism

 

Sequence read from 5′ end to 3′ end

Reference

18S

B. ostreae

Forward primer:

Reverse primer:

Probe:

CCCGGCTTCTTAGAGGGACTA

ACCTGTTATTGCCCCAATCTTC

FAM-CTGTGTCTCCAGCAGAT-BHQ1

Marty et al. (2006)23

ITS1

B. ostreae

Forward primer:

Reverse primer:

Probe:

CCCTGCCCTTTGTACACACC

TCACAAAGCTTCTAAGAACGCG

FAM-GGTGAATTAGGTGGATAAGAGCGCT-BHQ1

Corbeil et al. (2006)12

ELF 1α

O. edulis

Forward primer:

Reverse primer:

Probe:

GTCACGGACAGCAAAACGTC

TCGATTGCCACACTGCTCAT

FAM-GGTGAATTAGGTGGATAAGAGCGCT-BHQ1

Present study

IAC

IAG52B

Forward primer:

Reverse primer:

Probe:

CCAGTGTATCGCCTGTCAGG

ACTGGGTGAAGGTGGGAGAT

CY5-GGCGGTGCCGGCAGGACACAGG-BHQ2

Present study

  1. An internal amplification control assay (IAC) designed in the present study consisted of a plasmid with an artificial IAG52B construct and a CY5 labelled probe. Thus, this IAC was used in our assays using FAM labelled probes to detect inhibition in each sample.
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