Table 2 The average Ct values from qPCR results analysing genes 18S and ITS1 from Bonamia ostreae from 10 water samples (2L) from tanks with flat oysters from area 35.

From: A non-lethal method for detection of Bonamia ostreae in flat oyster (Ostrea edulis) using environmental DNA

Pore size of filter

 

Oyster number

1

2

3

4

5

6

7

8

9

10

1.2 µM

ITS1

–

35.2

–

–

–

–

–

–

–

–

18S

–

33.2

–

–

–

–

–

–

–

35.9

ELF1α

31.9

28.2

29.8

32.8

32.4

30.9

32.8

30.3

–

33.3

10 µM

ITS1

–

32.5

–

–

–

–

–

–

36.3

–

18S

35.3

34.7

–

33.8

31.5

–

–

–

34.2

–

ELF1α

27.8

25.5

26.8

27

29.4

28.1

28.2

27.8

26.3

27.3

  1. Water samples were filtrated using filters with pore sizes of first 10 µM and then 1.2 µM. Threshold was set to 37, thus all Ct values below 37 were regarded as positive for B. ostreae. “No Ct” values are indicated by a minus (−). The Ct value of the positive control was ~ 30 for ITS1, ~ 28 for 18S and ~ 29 for ELF 1α. Values in italics represent samples, where one qPCR reaction resulted in “No Ct” value and the other a Ct value below 37. These samples were regarded as under suspicion of including B. ostreae eDNA.
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