Figure 1

Optimization of Agrobacterium-mediated nuclear transformation of stevia. (A) Effect of explant type, (B) Concentration of acetosyringone (µm), (C) O.D₆₀₀ of Agrobacterium co-cultivation medium, (D) Pre-incubation duration (days), (E) Agro-inoculation duration (min) and (F) Co-cultivation duration (days).