Table 2 Stevia transformation (callus explant) and selection on glufosinate supplemented medium.
I selection cycle | II successive selection cycle | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Explant | Number of explants used (A) | Number of responding explants (B) | % response (B/A) | Number of shoots produced | Number of explants used (C) | Number of responding explants (D) | % response (D/C) | Herbicide resistant plants produced | % transformation efficiency | |
Callus | 80 | 15 | 18.75 | 50 | 80 | 22 | 27.50 | 06 | 27.27 | 27.94 ± 5.75 |
80 | 10 | 12.50 | 58 | 98 | 39 | 39.79 | 12 | 31.42 | ||
80 | 20 | 25.00 | 45 | 74 | 20 | 27.02 | 04 | 21.42 | ||
