Figure 8
Cachectic mice develop IL-1R-dependent liver and skeletal muscle fibrosis. (a–c) Western blot for alpha smooth muscle actin (⍺-SMA) or GAPDH on liver (a), gastrocnemius (b), and vWAT (c) tissue lysate at 9 wpi. Representative of at least 2 independent experiments. Each lane represents an individual mouse. The intensity ratio of ⍺-SMA to GAPDH is listed below each set of bands. n.d. not detectable. These images were cropped from full-length blots, which are presented in Supplementary Fig. 7. (d–f) Picrosirius red staining on formalin-fixed tissue at 9 weeks post-infection. Picrosirius red staining was quantified in ImageJ (below) and pixel density represented as % of each field of view (each point is 5 fields of view averaged per mouse). (d) Liver, N = 6–8 mice per group, pooled between 2 experiments. (e) Gastrocnemius, N = 6–7 mice per group, pooled between 2 experiments. (f) vWAT. N = 6–9 mice per group, pooled between 3 experiments. Error bars are standard error of the mean. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001 by unpaired Student’s t test.
