Table 1 Statistical results of MiSeq Illumina deep sequencing. Clean bases: total number of clean bases; Q20 (%)/Q30 (%): The percentage of nucleotides with a quality higher than 20 or 30/nucleotide (clean read1, read2); GC (%): Percent GC/nucleotide (clean read, read2).
From: Metagenomic analysis and identification of emerging pathogens in blood from healthy donors
Sample | Clean reads | Clean bases | Strategy | Q20 (%) | Q30 (%) | GC (%) |
|---|---|---|---|---|---|---|
DNA pool | 1,375,023,000 | 2,967,242 | PE150 | 99.48 | 99.15 | 40.9 |
cDNA pool | 2,082,710,000 | 3,450,046 | PE150 | 99.30 | 98.83 | 39.87 |