Figure 1

Localisation of dog MRGPR family genes. RT-qPCR analysis was performed to determine MRGPR family gene expression in various canine tissues. Relative gene expression level was calculated by 2−ΔΔCt method, using GAPDH as internal control. The ΔΔCt was calculated by subtracting the ΔCt of MRGPRD in the heart from the ΔCt of each sample. Data are expressed as the mean ± SD of three animals, except for the mean of two animals (*) or individual data of one animal (**) because of Ct > 40 or non-specific amplification.