Figure 3 | Scientific Reports

Figure 3

From: Identification of the dog orthologue of human MAS-related G protein coupled receptor X2 (MRGPRX2) essential for drug-induced pseudo-allergic reactions

Figure 3

Various histamine-releasing agents activate dog MRGPRX2 and human MRGPRX2 expressed in HEK293 cells. HEK293 cells transiently transfected with dog MRGPR family genes (D, F, G, or X2) or human MRGPRX2 were exposed to compound 48/80 or fluoroquinolones (ciprofloxacin [CPFX], gatifloxacin [GFLX], levofloxacin [LVFX], and pazufloxacin [PZFX]). (a) Increase in intracellular Ca2+ levels in a concentration dependent manner. Data represent the mean of quadruplicate assays. Non-transfected HEK293 cells were used as a negative control. (b) Time-course changes of intracellular Ca2+ levels in dog or human MRGPRX2-expressing HEK293 cells. Traces show representative intracellular Ca2+ fluctuation following exposure to compound 48/80 (10 μg/mL), CPFX (333 μg/mL), and PZFX (333 μg/mL). Test articles were perfused from 10 s. RLU Relative light units, s second.

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