Figure 3

NAD⁺-dependent deacylation activities in various cell lines. (a) NAD⁺-dependent desuccinylase activity, and (b) NAD⁺-dependent deacetylase activity in multiple cell lines. 200 µg of cell lysates, 10 µM of succinyl/acetyl-substrate and 500 µM NAD⁺ were present in the desuccinylation/deacetylation assays. Fluorescence signal obtained in the presence of substrate and absence of NAD⁺ was subtracted from each experiment sample. Experiments were done in duplicates, and error bars represent deviation from the mean.