Table 4 Descriptive correlation of HLA-G and HLA-F isoforms with low and high levels of expression and pathological complete response status in the subtypes.

From: HLA-G and HLA-F protein isoform expression in breast cancer patients receiving neoadjuvant treatment

 

HER2+ (n = 28)

Luminal B-like (n = 49)

Triple-negative (n = 38)

No pCR

pCR

No pCR

pCR

No pCR

pCR

Low HLA-F1

5 (36%)

9 (64%)

19 (79%)

5 (21%)

10 (53%)

9 (47%)

High HLA-F1

5 (36%)

9 (64%)

22 (88%)

3 (12%)

9 (47%)

10 (53%)

Low HLA-F3

5 (33%)

10 (67%)

21 (84%)

4 (16%)

11 (65%)

6 (35%)

High HLA-F3

5 (42%)

7 (58%)

20 (83%)

4 (17%)

8 (38%)

13 (62%)

Low HLA-G1

6 (46%)

7 (54%)

19 (86%)

3 (16%)

9 (45%)

11 (55%)

High HLA-G1

4 (29%)

10 (71%)

20 (83%)

4 (17%)

8 (50%)

8 (50%)

Low HLA-G2

7 (32%)

15 (68%)

33 (85%)

6 (15%)

17 (55%)

14 (45%)

High HLA-G2

3 (50%)

3 (50%)

8 (80%)

2 (20%)

2 (29%)

5 (71%)

Low HLA-G3

6 (35%)

11 (65%)

21 (88%)

3 (12%)

7 (47%)

8 (53%)

High HLA-G3

4 (36%)

7 (64%)

18 (78%)

5 (22%)

11 (50%)

11 (50%)

Low HLA-G5

4 (36%)

7 (64%)

20 (83%)

4 (17%)

12 (57%)

9 (43%)

High HLA-G5

6 (35%)

11 (65%)

18 (82%)

4 (18%)

7 (41%)

10 (59%)

Low HLA-G6

2 (14%)

12 (86%)

21 (88%)

3 (12%)

13 (68%)

6 (32%)

High HLA-G6

7 (54%)

6 (46%)

20 (80%)

5 (20%)

6 (32%)

13 (68%)

  1. Protein bands from Western blots were quantified using ImageJ. GAPDH was used as an internal protein control, and placental tissue was used as a reference sample for each blot. The isoform expression pattern was determined semiquantitatively by normalizing to GAPDH relative to the reference sample (HLA isoform/GAPDH ratio). The frequencies of high and low levels of expression were determined using the median of the HLA isoform/GAPDH ratio.
  2. HLA human leukocyte antigen, HER2 human epidermal growth factor receptor 2, pCR pathological complete response.