Figure 5

Prognostic transcriptome signature of conjunctival melanoma (CM). (A) Workflow of analysis. DEG between melanoma with poor and good clinical outcome were used to define a prognostic signature. Clinical outcome was defined based on the presence or absence of local recurrence and/or systemic metastases with a follow up of at least 24 months. (B) Heatmap of expression data of 20 prognostic marker genes of CM with good (orange) and poor outcome (red). Each row represents one gene and each column one tumor. The z-score represents a gene’s expression in relation to its mean expression by standard deviation units (red: upregulation, blue: downregulation). The genes are ordered according to their correlation with poor outcome, placing the gene with the highest correlation coefficient at the top (correlation coefficients are shown beside the heatmap). Tumors in the columns are ordered according to the correlation between the expression values of all 20 signature genes of one sample to the mean expression values of the other samples of both outcomes using leave-one-out validation (see methods). The correlation coefficients for both outcomes are shown below the heatmap and in (C): The white space around the diagonal is defined by the standard deviation of the distances of each sample from the diagonal. A sample that lies within the colored area can be assigned to an outcome with high probability.