Figure 6
From: Extra-mitochondrial mouse frataxin and its implications for mouse models of Friedreich’s ataxia
LC-PRM/MS analysis of human heart mature frataxin and human mature SILAC-frataxin (81-210) tryptic peptides (A). S81GTLGHPGSLDETTYER97 and S81GTLGHPGSLDETTYER97. (B) L136GGDLGTYVINK147 and L136GGDLGTYVINK147. (C) Q150IWLSSPSSGPK161 and Q150IWLSSPSSGPK161. (D) Relative amounts of frataxin proteoforms in human heart (n = 5) and mouse heart (n = 3). L = [13C615N1]-leucine, K = [13C615N2]-lysine. PRM/MS ions used for quantification of tryptic peptides were: S81GTLGHPGSLDETTYER97 y11+, y142+, and y132+; L136GGDLGTYVINK147, y11+, y7+, and b4+; Q150IWLSSPSSGPK161 y10+, y9+, and y8+. Accurate masses that were monitored are shown on the chromatograms.
