Figure 2
Coronal slices zooming into fetoplacental units, exemplifying the images and ADC maps derived from the multi-shot SPEN sequence in Fig. 1a, for different mice models. Data were collected on 1 mm slices with a 125 µm in-plane resolution using a 23 × 23 mm2 FOV. Additional parameters: TR/TE = 2000/32 ms, respiratory trigger, 5 interleaved shots, 8 averages per interleave, δ = 3.2 ms, ∆ = 10 ms, diffusion gradient = 33 G/cm (nominal b-value ≈ 750 s/mm2), three orthogonal directions, total scan time ~ 6 min. Red arrows highlight multi-layered placentas; blue arrows fetal brains; the rapidly-diffusing regions (intense red) in the ADC maps reflect the amniotic fluid. The l-NAME injected mouse was subject to two treatments prior to scanning on days E14.5 and E15.5, and hence E16.5 was the first day were differences versus wildtypes could be gathered.
