Table 1 Mutation, knock-in, and mosaicism rate in blastocysts after cytoplasmic injection of the gRNA:Cas9 RNP complex and hmejSRYp or hrSRYp in the MII oocyte or presumptive zygotes 6 h post insemination.

Female

MII

HMEJ

960

8.5 (82)

82.9 (68)

41.5 (34)^a

9.8 (8)^a

17.6 (6)^a

5.9 (2)^a

76.5 (26)^a

HR

125

11.2 (14)

78.6 (11)

28.6 (4)^b

14.3 (2)^a

50.0 (2)^a

0.0 (0)^a

50.0 (2)^a

6 hpi

HMEJ

85

15.3 (13)

92.3 (12)

46.2 (6)^a

15.4 (2)^a

16.7 (1)^a

16.7 (1)^a

66.7 (4)^a

HR

95

16.8 (16)

87.5(14)

18.8 (3)^b

0.0 (0)^a

0.0 (0)^a

0.0 (0)^a

100.0 (3)^a

Male

MII

HMEJ

1005

11.4 (115)

74.8 (86)

33.0 (38)^a

14.8 (17)^a

21.1 (8)^a

23.7 (9)^b

55.3 (21)^b

HR

125

12.8 (16)

81.3 (13)

6.3 (1)^b

0.0 (0)^a

0.0 (0)^a

0.0 (0)^a

100.0 (1)^b

6 hpi

HMEJ

85

20.0 (17)

94.1 (16)

35.3 (6)^a

23.5 (4)^a

16.7 (1)^a

50.0 (3)^b

33.3 (2)^b

HR

65

18.5 (12)

83.3 (10)

0.0 (0)^b

n/a

n/a

n/a

n/a

Total

MII

HMEJ

1965

10.0 (197)

78.2 (154)

36.5 (72)^a

12.7 (25)^b

19.4 (14)

15.3 (11)

65.3 (47)

HR

250

12.0 (30)

80.0 (24)

16.7 (5)^b

6.7 (2)^a

40.0 (2)

0.0 (0)

60.0 (3)

6 hpi

HMEJ

170

17.6 (30)

93.3 (28)

40.0 (12)^a

20.0 (6)^b

16.7 (2)

33.3 (4)

50.0 (6)

HR

160

17.5 (28)

85.7 (24)

10.7 (3)^b

0.0 (0)^a

0.0 (0)

0.0 (0)

100.0 (3)

Control

Not injected

2400

29.3 (702)

–

–

–

–

–

–