Figure 6
From: Nephronophthisis gene products display RNA-binding properties and are recruited to stress granules
Localization of BICC1 in response to hippuristanol. (a) HeLa cells were transfected with RFP-tagged BICC1, and either exposed to DMSO or 1 mM hippuristanol for 1 h. BICC1 accumulated in granules without co-localization with TIA-1 in the absence of stress. Addition of hippuristanol recruited BICC1 into TIA-1-positive stress granules. The experiment was performed four times, analyzing a total of 58 images and 15 z-stacks. (b) Co-transfection of GFP-AGO2 caused partial co-localization of BICC1 and AGO2 with TIA-1-positive granules. Hippuristanol exposure recruited most BICC1/AGO2-granules into TIA-1-positive stress granules. The experiment was performed four times, analyzing a total of 91 images and 25 z-stacks.
