Figure 7
From: Nephronophthisis gene products display RNA-binding properties and are recruited to stress granules
Localization of INVERSIN in response to hippuristanol. (a) HeLa cells were transfected with RFP-tagged INVERSIN, and either exposed to DMSO or 1 mM hippuristanol for 1 h. INVERSIN assumed a diffuse cellular localization. Upon treatment with 1 mM hippuristanol, INVERSIN accumulated in TIA-1-positive stress granules. The experiment was performed four times, analyzing a total of 72 images and 22 z-stacks. (b) Co-transfection of GFP-tagged AGO2 did not alter the localization of INVERSIN. However, hippuristanol exposure recruited AGO2/INVERSIN-granules into TIA-1-positive stress granules. The experiment was performed five times, analyzing a total of 67 images and 24 z-stacks.
