Figure 8
From: Nephronophthisis gene products display RNA-binding properties and are recruited to stress granules
BICC1-mediated recruitment of NPH proteins into stress granules. (a) Co-expression of BICC1 recruited ANKS3 into granules that co-localized with TIA-1 in response to 1 mM hippuristanol. The experiment was performed two times, analyzing a total of 18 z-stacks. (b) Co-expression of BICC1 recruited ANKS6 into granules that co-localized with TIA-1 in response to 1 mM hippuristanol. The experiment was performed two times, analyzing a total of 17 z-stacks. (c) GFP-tagged INVERSIN and RFP-tagged BICC1 co-localized in granules that overlapped with TIA-1-positive stress granules even in the absence of hippuristanol. Hippuristanol treatment (1 mM for 1 h) increased the number of TIA-1-positive stress granules that largely co-localized with INVERSIN and BICC1. In this experiment, a total of 19 z-stacks were analyzed.
