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Figure 1

From: Dimethyl 3,3′-dithiobispropionimidate-functionalized diatomaceous earth particles for efficient biomolecule separation

Figure 1

Schematic representation of the principle of the DTBP-based AD in filter system for biomolecule separation. Schematic diagram of the functionalization by APDMS on a DE surface (Step 1). The sample lysate is mixed with DTBP and AD; then, the lysate is incubated to capture DNA via the DTBP on the amine-modified AD surface (Step 2). After this incubation, the protein fractions are directly separated from the single-filter without any additional instruments or detergents (Step 3). Finally, the DNA is isolated from the system with an elution buffer (10 mM sodium bicarbonate, pH 10.6.) (Step 4). (DE: diatomaceous earth, AD: APDMS-functionalized DE).

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