Figure 5 | Scientific Reports

Figure 5

From: Asymmetric dimethylarginine (ADMA) accelerates renal cell fibrosis under high glucose condition through NOX4/ROS/ERK signaling pathway

Figure 5

ADMA augments myofibroblast activation through NOX4: (A,B) Cells were pretreated with DPI(D) for 30 min before HG(H)/ADMA(A) treatment and were harvested after 24 h. NOX4, fibronectin, α-SMA were detected by immunoblot with GAPDH as a loading control. (C,D) Cells were transfected with NOX4 SiRNA(Si)/Scramble RNA(Scr). Two days after the transfection, cells were treated with HG(H)/ADMA(A) and were harvested after 24 h treatment. NOX4, fibronectin, α-SMA were detected by immunoblot with GAPDH as a loading control. Band intensity was calculated using the ImageJ software. Results are presented as Mean ± SD of three independent experiments. *p < 0.05 compared to the Control, #p < 0.05 compared to the HG, $p < 0.05 compared to the ADMA. Original uncropped images of the blots are given in Supplementary Fig S8.

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