Figure 6 | Scientific Reports

Figure 6

From: Asymmetric dimethylarginine (ADMA) accelerates renal cell fibrosis under high glucose condition through NOX4/ROS/ERK signaling pathway

Figure 6

ADMA induced myofibroblast activation is characterized by stimulation of ERK1/2 pathway: cells were treated with ADMA in (A) dose and (B) time dependent manner and were harvested after respective conditions. p-ERK was detected by using immunoblot with t-ERK as loading control. (C,D) Cells were pretreated with U0126(U) for 30 min before HG(H)/ADMA(A) treatment and were harvested after 24 h. p-ERK, fibronectin and α-SMA were detected by immunoblot. Band intensity was calculated using the ImageJ software. Results are presented as Mean ± SD of three independent experiments. (A) *p < 0.05 compared with Control, #p < 0.05 compared with ADMA (10 μM), $p < 0.05 compared with ADMA (20 μM). (B) *p < 0.05 compared with Control, $p < 0.05 compared with 30′, #p < 0.05 compared with 1 h. (C,D) *p < 0.05 compared to the Control, #p < 0.05 compared to the HG, $p < 0.05 compared to the ADMA. Original uncropped images of the blots are given in Supplementary Fig. S9.

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