Figure 7

ADMA regulates NOX4/ROS/ERK signaling pathway during the high glucose induced myofibroblast activation: (A,C) Cells were pretreated with GW4064(G) for 30 min before HG(H)/ADMA(A) treatment and were harvested after 24 h. (B) Cells were pretreated with l-Arginine (ARG/AR) for 30 min before HG/ADMA treatment and were harvested after 24 h. (D) Cells were transfected with NOX4 SiRNA (N Si)/Scramble RNA (Scr). Two days after the transfection, cells were treated with HG(H)/ADMA(A) and were harvested after 24 h treatment. (E) Cells were pretreated with U0126(U) for 30 min before HG(H)/ADMA(A) treatment and were harvested after 24 h. p-ERK and NOX4 of respective conditions were detected by using immunoblot with t-ERK and GAPDH as loading control respectively. Band intensity was calculated using the ImageJ software. Results are presented as Mean ± SD of three independent experiments. *p < 0.05 compared to the Control, ^#p < 0.05 compared to the HG, ^$p < 0.05 compared to the ADMA. Original uncropped images of the blots are given in Supplementary Fig. S10.