Figure 2 | Scientific Reports

Figure 2

From: Ambrisentan, an endothelin receptor type A-selective antagonist, inhibits cancer cell migration, invasion, and metastasis

Figure 2

Ambrisentan inhibits migration and invasion capacity of MDA-MB-231 cells without any impact on cell viability. (a) Flow cytometry histogram shows the extent of ETAR expression by MDA-MB-231 cells. (b) Impact of Ambrisentan on cellular vaibility of exponentially growing MDA-MB-231 cells treated with either vehicle (0.1% DMSO) or the indicated concentrations of Ambrisentan for 24 h. (c, d) Viable cells that were able to cross the 8-mm pores insert (migration assay; c) and the matrigel matrix (invasion assay; d) were quantified using the CellTiter-Glo luminescent cell viability assay. The data are expressed as means ± SD of 2–3 replicates per group and are pooled from 3 independent experiments. Asterisks denote statistically significant differences between Ambrisentan-treated cells compared to controls (***p < 0.001; ****p < 0.0001).

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