Figure 7 | Scientific Reports

Figure 7

From: Development of droplet digital PCR for the detection of Tilletia laevis, which causes common bunt of wheat, based on the SCAR marker derived from ISSR and real-time PCR

Figure 7

Statistical analysis by ddPCR (A) Positive copy number analysis for detection of T. laevis by copy number; 1–3, DNA template of T. laevis (30 ng/µl); 4–6, DNA template of T. laevis (3 ng/µl); 7–9, DNA template of T. laevis (0.3 ng/µl); 10–12, DNA template of T. laevis (30 fg/µl). (B) Number analysis of T. laevis isolates; 1–3, DNA template of T. laevis (30 ng/µl); 4–6, DNA template of T. laevis (3 ng/µl); 7–9, DNA template of T. laevis (0.3 ng/µl); 10–12, DNA template of T. laevis (30 fg/µl). Gray pillars are positive droplets, and blue pillars are total droplets (positive + negative).

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