Figure 1

Transfection efficiency of BMDCs. BMDCs were transfected with 50 nM of FAM-labeled miR-369-3p mimic and unlabeled miR-369-3p mimic. (A) Representative images of BMDCs transfected with FAM-labeled miR-369-3p mimic. Bright‑field (left) and fluorescence (right) images for mock condition and transfected condition were acquired using fluorescence microscopy. Original magnification, × 20. Scale bar presents 50 μm. (B) miR-369-3p expression levels in transfected BMDCs. miR-369-3p levels were determined by qRT-PCR and normalized to the geometric mean of two endogenous controls (miR-186-5p and miR-26a-5p). Data are shown as the means ± SEM.