Figure 10
Stimulated reversal speed and age-associated locomotory decline in wild-type C. elegans. (a) A representative locomotion episode of a day-5 C. elegans over 40 s duration. Each filled circle represents instantaneous speed between two consecutive frames determined by manually tracking the vulva of the animal. Tap stimulus was applied (frame # 10, red arrow) to induce reversal. Animal executed reversal with a very high speed followed by second reversal. Animal turns (blue arrow), crawls in forward direction (black brackets) for relatively long time with pause (amber bracket). Naturally occurring reversal period is shown by the pink bracket. Average speed of the full episode, only forward (including pauses), only reversal and stimulated reversal are shown in the inset. Error bars are standard deviations. n = 5. (b) Decline in forward and first reversal speed as wild type C. elegans ages in the micropillar arena. Error bar is the standard deviation. n > 10, food: 100 mg E. coli OP50/mL S complete, feeding frequency: once/day.
