Figure 4
Optimization of feeding protocol for C. elegans lifespan assays. (a) Lifespan of wild type C. elegans for feeding frequency of twice per day (n = 112), once per day (n = 87) and every other day (n = 92). N = 2 repeat trials. Food concentration: 100 mg/mL of E. coli OP50 in S-complete. (b) Lifespan of wild type C. elegans for food concentration of 100 mg/mL (n = 87) and 200 mg/mL (n = 74) of E.coli OP50 in S-complete. N = 1 trial. Feeding frequency: once every day. Feeding animals every other day, produces a lower median (and mean) and higher maximal lifespan than feeding every day (p = 0.0036) or twice per day (p < 0.001). p value (once per day vs twice per day) = 0.0244.
