Table 1 Influence of population density and number of animals per chamber on lifespan of wild-type animals.

From: NemaLife chip: a micropillar-based microfluidic culture device optimized for aging studies in crawling C. elegans

Area (mm2) per animal

Animals per chamber

Number of trials

Animals scored per trial

Median lifespan, day

Statistical significance

4.0–6.0

10–15

5

86–100

13.6 ± 0.56

–

7.0

1

4

59–91

14.2 ± 0.75

n.s

10–12

60–70

2

60–70

13.5 ± 0.71

n.s

  1. Statistical comparison of survival curves were made with respect to our standard assay condition (highlighted in bold) using log-rank test. p value of > 0.05 corresponds to not significant (n.s.).
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