Figure 6
Bioluminescent aequorin reporter assays for mAChR-A expressed in CHO-K1 cells alongside the human Gα15(16) subunit. (A) Dose–response curves to various doses of acetylcholine (ACh), muscarine (MUS), and pilocarpine (PIL). (B) Representative 20-s typical luminescent responses to different doses of ligand (muscarine in this case). Inset in (B) shows the integrated relative luminescent values calculated from 40 intervals within the 20 s responses. (C) Luminescent responses to different drugs at 1 μM concentration. Dopamine (DA), octopamine (OCT), and epinephrine (EPI). (D) 20-s luminescent responses to 1 μM drugs. (E) Typical dose–response curve of antagonistic atropine activity. (F) Representative luminescent responses to 10 μM ACh after pre-incubation with different atropine concentrations. The bars in (A,C,E) indicate the standard error for three replicates. In (A,E) some standard error bars are smaller than the symbols used and in these cases, only the symbols are shown.
