Figure 7
Effects of vesamicol and atropine on volume of water ingested by desiccated I. ricinus females. (A) Experimental set-up of forced water ingestion by desiccated ticks. Four different groups of Ixodes females (upper panel) were connected to glass microcapillary tubes (arrow, total volume 1 μl) via their hypostomes (lower panel). The black square shows the double-sided adhesive tape to which the ticks were attached upside-down. Inset in lower panel shows detail of the hypostome inserted into the glass microcapillary. (B) Ingested water volume by ticks pre-injected with PBS or different drugs at 30 min. (C) Ingested water volume by the same group of ticks as in (B) at 60 min. Each symbol in (B,C) indicates an individual Ixodes female. The horizontal dotted line represents the mean, and the bars the standard deviation. The experiment represents two biological replicates.
