Figure 3 | Scientific Reports

Figure 3

From: Characterization of five novel vasopressin V2 receptor mutants causing nephrogenic diabetes insipidus reveals a role of tolvaptan for M272R-V2R mutation

Figure 3

Response to dDAVP treatment. (A) cAMP generation in COS-7 cells expressing WT, Mock and NDI-causing V2R mutants. cAMP levels in COS-7 expressing WT and mutant V2R were compared with cAMP in Mock-expressing COS-7 cells. Values are means ± SEM (n = 3, ***P < 0.001, one-way ANOVA). (B) dDAVP-induced V2R internalization. Representative pictures of V2R-GFP (green) and nuclei stained by DAPI (red) revealed a proper resposnse to dDAVP only in cells expressing WT-GFP V2R.

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